Oct 18, 2024
Malt extract broth and agarized
- Marta Puddu1
- 1Polimi
Protocol Citation: Marta Puddu 2024. Malt extract broth and agarized. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lywmwevx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 17, 2024
Last Modified: October 18, 2024
Protocol Integer ID: 110195
Funders Acknowledgement:
MP
Abstract
Recipe and protocol for Malt extract broth medium preparation. An optional step for agarization is provided.
Recipe and steps to prepare your mixture
Recipe and steps to prepare your mixture
Take note of the position of YourFavouriteIngredients and take them from the reagent-room.
Weight ingredients:
Tryptone/Peptone powder (technical grade) 5 g/L
Yeast extract 3 g/L
Malt extract (technical grade) 3 g/L
Glucose (technical grade) 10 g/L
Milli Q or Tap water to volume 1L.
Add all the solid ingredients to a plastic or glass becker, along with 600-700mL of water to dissolve the ingredients.
Its facultative to measure Water with a cilinder.
Its facultative to add autoclavable components (vitamins, antibiotics etc.)
Agarization
Agarization
Add 15 g/L Agar microbiology grade along with 100mL of water to "dissolve" the agar. It will settle to the bottom due to low solubilization below 50°C, it will be mixed during autoclaving step.
Finalise your mixture
Finalise your mixture
Make up to final 1L volume with remaining water and further mix.
Autoclave it!
Autoclave it!
Pour the content into a Duran to autoclave the mixture.
Do not fill the whole bottle, leave some headspace (1/4 of total volume HS).
Do not seal the bottle, leave the top half-round open.
Remember to place autoclave-tape over your bottles.
Autoclave setting: 121°C for 15min, 15psi/1bar