Oct 20, 2023
Magnetic resonance imaging (MRI)
- 1Vall d'Hebron Research Institute
Protocol Citation: Núria Peñuelas, Ariadna Laguna, Miquel Vila 2023. Magnetic resonance imaging (MRI). protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6x3mdlqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 19, 2023
Last Modified: November 17, 2023
Protocol Integer ID: 89600
Abstract
Magnetic resonance imaging (MRI) of mouse brain
Prepare the equipment: 7T Bruker BioSpec 70/30USR scanner (Bruker BioSpin GmbH, Ettlingen, Germany) equipped with a mini-imaging gradient set (400mT/m) and using a 72-mm inner diameter linear volume coil as a transmitter and a dedicated mouse brain surface coil as a receiver.
Prepare the software: Acquire and process all MRI data on a Linux computer using Paravision 5.1 software (Bruker BioSpin GmbH, Karlsruhe,Germany)(RRID:SCR_001964, http://www.bruker.com/service/support-upgrades/software-downloads/mri.html).
Anesthetize the animals (1.5-2% isoflurane in 1 L/min oxygen for maintenance) and place them into an animal bed with bite-bar and ear-bars for optimal head immobilization.
Control the respiration rate (50-100 bpm) and core body temperature (37 ± 1°C) with an animal monitoring and control system (SA Instruments, Stony Brook, NT).
Acquire high resolution T1-weighted spin-echo images in the axial plane with and without fat saturation pulses.
Acquire two slice packages of 7 contiguous slices each containing the SNpc and LC regions and using the following parameter: TE=7 ms; TR= 500 ms; NA=56; FOV=1.92×1.92 cm2; MTX=128×128; ST= 0.25 mm; thus, resulting in a spatial resolution of 150×150×250 µm3; Acqt= 1h per image.