License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 31, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 67954
Keywords: ASAPCRN
Abstract
This protocol describes a method to perform calcium imaging with Fura-2 (live-cell imaging)
Cells are washed with HBSS (Cat No. 14025092, ThermofisherScientific)
Cells are loaded with 5uM of Fura 2-AM (F1221, ThermofisherScientific) in HBSS.
CCD protocol is set up by selecting a501 nm wheel (both 340 nm and 380 nm wavelength are excited b 501 nm).