Pick one yeast colony from the plate or spin down 100-200 μl of liquid yeast culture (OD600=0.4). Suspend cells in 100 μl of 200mM LiOAc, 1 % SDS solution. Alternatively, you can spin down 100 - 500 uL of liquid culture at 3,000 rcf for 4 minutes and then resuspend the pelleted cells in 100 uL of 200 mM LiOAc, 1% SDS solution if you wish.