Lipid droplets are organelles involved in intracellular lipid homeostasis, as well as playing key roles in a variety of cellular functions including cellular signalling, metabolic disease and inflammation. BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) is a fatty acid-conjugated fluorescent probe that has been shown to successfully visualise the localisation and dynamics of lipid droplets in cells. Analysis can be performed on cells cultured in complete growth medium or in cells following lipid loading with oleic acid (OA) after over-night starvation in OptiMEM. OA is a potent inducer of lipid droplet formation.