Apr 14, 2023
Version 1
Lemnaceae (Duckweed) culturing protocol V.1
This protocol is a draft, published without a DOI.
- 1University of Toronto
Protocol Citation: o.pogoutse, Jason Laurich, Chris Carlson 2023. Lemnaceae (Duckweed) culturing protocol. protocols.io https://protocols.io/view/lemnaceae-duckweed-culturing-protocol-bz6ap9ae
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 17, 2021
Last Modified: April 14, 2023
Protocol Integer ID: 55202
Abstract
Protocol for culturing duckweed.
Materials
1000 ml beaker
5x YMA
CaCl2
5 Falcon tubes per line to culture
70% Ethanol In spray bottle
Pipetteman pipetter
Sterilisation and Setup
Sterilisation and Setup
Spray down inside of biosafety cabinet with ethanol, including materials
to be used.
YMA Media Preparation
YMA Media Preparation
To prepare 500ml of 1x YMA media in the biosafety cabinet pour 100 ml of sterile
5x YMA media into sterile cylinder.
Next add sterile H2O till the beaker contains around 350 ml.
Next add 75 ml of CaCl2 stock solution (sterile stock 1.5mg/ml).
Top off with sterile H2O to 500 ml total volume.
Add 500 μL of 1000x stock solution of MgSO4 x 7H2O (150mg/ml, filter sterilized) to media. Transfer prepared media to a sterile bottle and mix by inverting the bottle a few times.