May 16, 2024
Version 2
Leica SP8 Confocal Imaging V.2
- Allen Institute for Brain Science1
- 1Allen Institute
Protocol Citation: Allen Institute for Brain Science 2024. Leica SP8 Confocal Imaging. protocols.io https://dx.doi.org/10.17504/protocols.io.261geo2wdl47/v2Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 25, 2020
Last Modified: May 16, 2024
Protocol Integer ID: 99995
Keywords: leica, MC0141, z stack, z-stack, imaging, confocal,
Abstract
This protocol describes the basic setup and scanning using the Leica SP8 confocal microscope of fluorescently-labeled mouse brain tissue sections mounted on positively charged 1” x 3” microscope slides.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
