Jun 21, 2023

Public workspaceLB Broth

DOI
dx.doi.org/10.17504/protocols.io.yxmvm375nl3p/v1
  • 1University of Sheffield;
  • 2Sheffield iGEM
sgoodwin
Open access
DOI: dx.doi.org/10.17504/protocols.io.yxmvm375nl3p/v1
Protocol Citation: sgoodwin 2023. LB Broth. protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvm375nl3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: June 21, 2023
Last Modified: June 21, 2023
Protocol Integer ID: 83812
Abstract
Process for producing LB broth growth medium
Making the LB broth
Making the LB broth
3h 10m
3h 10m
Dilute Amount20 g of LB powder in Amount800 mL distilled water

5m
Stir until fully dissolved with magnetic stirrer
5m
Transfer to a Duran flask, and sterilise by autoclaving
3h