Jun 21, 2023

Public workspaceLB Agar

DOI
dx.doi.org/10.17504/protocols.io.dm6gp38xjvzp/v1
  • 1Sheffield iGEM
sgoodwin
Open access
DOI: dx.doi.org/10.17504/protocols.io.dm6gp38xjvzp/v1
Protocol Citation: sgoodwin 2023. LB Agar. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp38xjvzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: June 21, 2023
Last Modified: June 21, 2023
Protocol Integer ID: 83813
Abstract
Protocol for producing LB agar growth medium
LB Agar
LB Agar
3h 11m
3h 11m
Add Amount1.5 g LB powder, Amount1.5 g agarose powder to conical flask

5m

Dilute with Amount200 mL distilled water. Ensure fully dissolved

5m
Seal conical flask with sponge bung and foil
1m
Sterilise growth medium by autoclaving
3h