Dec 12, 2019
Lactophenol-aniline blue solution
- 1University of Georgia
- Mimulus
Protocol Citation: Andrea Sweigart 2019. Lactophenol-aniline blue solution. protocols.io https://dx.doi.org/10.17504/protocols.io.baf4ibqw
Manuscript citation:
Kearns, CA and Inouye, DW. Techniques for Pollination Biologists.University Press of Colorado.1993. pp. 111–111.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 12, 2019
Last Modified: December 13, 2019
Protocol Integer ID: 30940
Keywords: pollen, staining, lactophenol, blue
Abstract
Lactophenol-aniline blue recipe
Guidelines
Wear gloves and prepare in the hood.
Materials
MATERIALS
Water
Phenol
Glycerine/Glycerol
Lactic Acid (85% v/v)
1% aqueous aniline blue
STEP MATERIALS
Water
Lactic Acid (85% v/v)
Phenol
Glycerine/Glycerol
1% aqueous aniline blue
Protocol materials
Lactic Acid (85% v/v)
In Materials, Materials, Step 1
Glycerine/Glycerol
In Materials, Materials, Step 1
1% aqueous aniline blue
In Materials, Materials, Step 2
Phenol
In Materials, Materials, Step 1
Water
In Materials, Materials, Step 1
Safety warnings
Please refer to the MSDS for further safety details
Make Lactophenol
Make Lactophenol
To make 100 mL of stock Lactophenol:
Combine:
20 mL
Phenol
40 mL
Glycerine/Glycerol
20 mL
Water
20 mL
Lactic Acid (85% v/v)
Lactophenol-aniline blue recipe
Lactophenol-aniline blue recipe
To prepare 100 mL working solution, combine:
100 mL stock Lactophenol
1 mL to 5 mL
1% aqueous aniline blue