Nov 12, 2024
L-DOPA Drug Treatment Alcacer et al 2024
- 1Alcala University
Protocol Citation: Cristina Alcacer 2024. L-DOPA Drug Treatment Alcacer et al 2024 . protocols.io https://dx.doi.org/10.17504/protocols.io.8epv52625v1b/v1
Manuscript citation:
Abnormal hyperactivity of specific striatal ensembles encodes distinct dyskinetic behaviors revealed by high-resolution clustering
Cristina Alcacer, Andreas Klaus, Marcelo Mendonça, Sara F. Abalde, Maria Angela Cenci, Rui M. Costa
bioRxiv 2024.09.06.611664; doi: https://doi.org/10.1101/2024.09.06.611664
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 31, 2024
Last Modified: November 12, 2024
Protocol Integer ID: 111377
Keywords: ASAPCRN, L-DOPA
Abstract
L-DOPA drug treatment administered to mice for imaging and behavioral studies, see protocol- "Open-field experiments from Alcacer et al 2024" DOI:
dx.doi.org/10.17504/protocols.io.6qpvr9e9bvmk/v1 is the protocol this treatment protocol is used in.
Materials
L-DOPA methyl ester (L-DOPA also called LD; from Sigma Aldrich, Portugal)
Peripheral DOPA decarboxylase inhibitor benserazide-HCl (Sigma Aldrich, Portugal)
Physiological saline (9 g/L NaCl), vehicle
Injection syringe
Before start
Dissolve L-DOPA methyl ester into physiological saline (9g/L NaCl) at a concentration of 6mg/kg final dosage and peripheral DOPA decarboxylase inhibitor benserazide-HCl at a concentration of 12mg/kg.
Physiological saline (9g/L NaCl) is used as the control/vehicle injections.
L-DOPA or Vehicle administration
L-DOPA or Vehicle administration
Inject vehicle intraperitoneally (i.p) for 2 consecutive days while performing either behavioral or imaging experiments.
On the 3rd day, inject the L-DOPA solution at 10mL/kg body weight or vehicle (pending treatment group) i.p. and proceed with behavioral or imaging recordings.
L-DOPA or Vehicle was then administered for a maximum of 4 days i.p. wile recording for either behavioral or imaging experiments at 6 mg/kg dosage.