Jan 24, 2019

Public workspaceKings B Media (1L)

DOI
dx.doi.org/10.17504/protocols.io.xf4fjqw
  • 1University of Arizona
Brian A Smith
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DOI: dx.doi.org/10.17504/protocols.io.xf4fjqw
Protocol Citation: Brian A Smith, Baltrus@Email.arizona.edu Micro.tucson 2019. Kings B Media (1L). protocols.io https://dx.doi.org/10.17504/protocols.io.xf4fjqw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use these protocols commonly in our group and they are working.
Created: January 24, 2019
Last Modified: January 24, 2019
Protocol Integer ID: 19676
Fill container aproximately 750mL of H2O
Fill container aproximately 750mL of H2O
Add stir bar and set to mix
Add Bacto Peptone
Add Bacto Peptone
Amount10 g Bacto Peptone
Amount10 g Bacto Tryptone
Amount10 mL Glycerol





Add K2HPO4
Add K2HPO4
Amount1.5 g
or
Amount10 mL of 1M Solution

Add MGSO4•H2O
Add MGSO4•H2O
Amount1.5 g
or
Amount10 mL of 1M Solution
Bring volume to 1L by adding H2O
Bring volume to 1L by adding H2O
Allow to mix again with stir bar.

Add Agar (if making plates)
Add Agar (if making plates)
Add agar to container to be autclaved
Amount15 g agar
Then add media