25 x 6-well plates (imaging plates)
- 3g NaCl (ref: fisher 447300010 )
- 2.5g bactopeptone (ref: BD 21167 lot 8270639)
- 17g agar (ref : A7002-5KG lot BCBM1702V)
- Salts (post-autoclave):
25mL KH2PO4, pH=6.0 (ref : SIGMA P0662-2,5KG)
1mL MgSO4, 7H2O (1M) (ref : SIGMA M5921-500G)
1mL CaCl2 (1M) (ref : SIGMA C5080-500G)
1mL cholesterol (5mg/mL) (ref : SIGMA C8667-5G)
- 25g LB powder (ref : fisher BP9723-500)
- 7g Na2HPO4.2H2O (ref : SIGMA 71645-1KG )
50mg/mL Kanamycin (in water, filter 0.2mm)
Antioxidants (Trolox, NAC and Vitamin C):
- Final concentration (in 4mL M9) : 500 and 1000 mg/mL
- Dilution in H2O, except Trolox [238813-1G sigma], which was diluted in DMSO
(pH for Trolox was adjusted with NaOH)