10 x 6-well plates (imaging plates)
10 x 60mm Petri plates (maintenance plates
3 x 90mm Petri plates (nursery plates)
- 3g NaCl (ref: fisher 447300010 )
- 2.5g bactopeptone (ref: BD 21167 lot 8270639)
- 17g agar (ref : A7002-5KG lot BCBM1702V)
- Salts (post-autoclave):
25mL KH2PO4, pH=6.0 (ref : SIGMA P0662-2,5KG)
1mL MgSO4, 7H2O (1M) (ref : SIGMA M5921-500G)
1mL CaCl2 (1M) (ref : SIGMA C5080-500G)
1mL cholesterol (5mg/mL) (ref : SIGMA C8667-5G)
- 25g LB powder (ref : fisher BP9723-500)
- 7g Na2HPO4.2H2O (ref : SIGMA 71645-1KG )
50mg/mL Kanamycin (in water, filter 0.2mm)
- Initial concentration: 1mg/mL
- Final concentration : 200mg/mL in 4mL M9
- (pH for Trolox adjusted with NaOH)
- Initial concentration: 100mg/mL 200mg in 2ml ethanol (in the freezer -20)
Intermediate concentration : 1mg/ml à in 100mL in 10ml M9 (filtered sterile)
Final concentration : 200mg/mlà in 4ml of M9 sterile, put 800ul of the intermediate solution (1mg/ml)
Put 200ul on plate (4ml NGM) to have a concentration of 10ug/ml
Trolox 238813-1G sigma (in DMSO first)
Trans resveratrol : PHR2201-200MG sigma (dilution in DMSO)
NAC, Vitamin C (dilution in water)