May 23, 2024

Public workspaceKAPP-Sen TMC: Xenium Pancreas FFPE Tissue Preparation

DOI
dx.doi.org/10.17504/protocols.io.kqdg32x5zv25/v1
  • 1Single Cell Biology Lab, The Jackson Laboratory, USA
Sergii Domanskyi
Open access
DOI: dx.doi.org/10.17504/protocols.io.kqdg32x5zv25/v1
Protocol Citation: Emily Soja, Shruti Bhargava, Santhosh Sivajothi, William F Flynn, Elise T Courtois 2024. KAPP-Sen TMC: Xenium Pancreas FFPE Tissue Preparation . protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg32x5zv25/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 22, 2024
Last Modified: May 23, 2024
Protocol Integer ID: 100292
Keywords: SenNet KAPP-Sen TMC
Funders Acknowledgement:
National Institute on Aging (NIA) KAPP-Sen Tissue Mapping Collaborative
Grant ID: U54 AG075941
Abstract
Xenium protocol for sectioning FFPE tissue blocks.
Xenium In Situ for FFPE - Tissue Preparation Guide CG000578 Rev C:Download CG000578_Demonstrated_Protocol_XeniumInSituProtocolsFFPE_TissuePreparationGuide_Rev_C.pdfCG000578_Demonstrated_Protocol_XeniumInSituProtocolsFFPE_TissuePreparationGuide_Rev_C.pdf8.3MB

The optimal water bath temperature for pancreas tissue was determined to be 38 C (see page 22 of CG000578 Rev C).