Aug 30, 2024
KAPP-Sen TMC: Whole human kidney preparation
- Juliana Alcoforado Diniz1,
- Ramalakshmi Ramasamy1,
- Alejandro Peña2,
- Qian Wu3,
- Jillian L. Woodworth2,
- Vladislav Bugay2,
- Manpreet Semwal2,
- Paul Robson1,4,5
- 1The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA;
- 2University of Texas Health Science Center at San Antonio, San Antonio, TX, USA;
- 3Department of Pathology and Laboratory Medicine, University of Connecticut School of Medicine, Farmington, CT, USA;
- 4Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, Farmington, CT, USA;
- 5Institute for Systems Genomics, University of Connecticut, Farmington, CT, USA
- Cellular Senescence Network (SenNet) Method Development Community
- KAPP-Sen TMC
Protocol Citation: Juliana Alcoforado Diniz, Ramalakshmi Ramasamy, Alejandro Peña, Qian Wu, Jillian L. Woodworth, Vladislav Bugay, Manpreet Semwal, Paul Robson 2024. KAPP-Sen TMC: Whole human kidney preparation. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyw4kwvx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 27, 2024
Last Modified: August 30, 2024
Protocol Integer ID: 106495
Keywords: KAPP-Sen TMC
Funders Acknowledgement:
National Institute on Aging (NIA) KAPP-Sen Tissue Mapping Collaborative
Grant ID: U54 AG075941
Abstract
This protocol describes removal, shipping, dissection, formalin preservation of a whole human kidney from a brain-dead donor.
Remove the whole kidney from a brain-dead donor. Flush the kidney with UW solution and perfuse with 1 liter of Waters IGL Pulsatile Perfusion Solution (PPS) for shipping.
Use overnight (ON) shipping, cold with ice.
Once received, bring the sample still on ice to the pathology department at UConn Health for dissection. The whole process was performed at room temperature (RT) and non-sterile.
Cut the whole organ in half along the middle plane (coronal section) and remove tissue pieces with a scalpel. The samples are roughly 1 cm x 1 cm and 0.5 cm thick and placed directly in labelled containers with 10 ml of 10% Neutral buffered formalin (NBF).
The samples are kept in RT and shipped the same day to JAX Histology Services in Bar Harbor. The samples are submitted to paraffinization (embedding of tissue blocks in paraffin) the same day they are received.