Sep 01, 2023
KAPP-Sen TMC: Preparation of Workspace for Tissue Blocks Sectioning
- Juliana Alcoforado Diniz1,
- Elaine Bechtel2,
- Paul Robson1,3,4
- 1The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA;
- 2The Jackson Laboratory, Bar Harbor, ME, USA;
- 3Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, Farmington, CT, USA;
- 4Institute for Systems Genomics, University of Connecticut, Farmington, CT, USA
Protocol Citation: Juliana Alcoforado Diniz, Elaine Bechtel, Paul Robson 2023. KAPP-Sen TMC: Preparation of Workspace for Tissue Blocks Sectioning. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgq37polk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 28, 2023
Last Modified: September 01, 2023
Protocol Integer ID: 85642
Keywords: SenNet KAPP-Sen TMC
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Abstract
Preparation of Workspace for Tissue Blocks Sectioning
Preparation of Workspace
Preparation of Workspace
Wear nitrile gloves cleaned with RNase Zap wipe.
Using RNase Zap wipes, clean microtome including any surface block or hands may contact (block holder, knife holder, handle, etc.).
Using RNase Zap wipes, clean glass Pyrex water bath insert and rinse with deionized water. Fill with deionized water and heat per usual.
For ice tray, using RNase Zap wipes, clean ice tray. For ice block, at least 24hrs prior to sectioning, using RNase Zap wipes, clean plastic ice container and rinse with deionized water. Fill with deionized water and put in -20°C freezer. To use, cover ice with enough deionized water that the blocks will not freeze to ice.
Using RNase Zap wipes, clean forceps and brushes/applicator sticks.
**This step will need to be repeated between each block.**
Using RNase Zap wipes, clean slide racks that will hold unstained slides.