Aug 09, 2023
KAPP-Sen TMC: Pancreas Tissue Blocks Paraffin Embedding
- Cristina Aguayo-Mazzucato1,
- Kanako Iwasaki1,2
- 1Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA;
- 2Tazuke Kofukai Medical Research Institute, Kitano Hospital, Osaka, Japan
Protocol Citation: Cristina Aguayo-Mazzucato, Kanako Iwasaki 2023. KAPP-Sen TMC: Pancreas Tissue Blocks Paraffin Embedding. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgq3j1olk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 09, 2023
Last Modified: August 09, 2023
Protocol Integer ID: 86242
Keywords: SenNet KAPP-Sen TMC
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Abstract
The paraffin cassettes were fixed in 4% paraformaldehyde for 24 hours and processed for paraffin embedding at Joslin Histology Core as described here.
Tissue processing using the Citadel 2000 Table Top Processor
Tissue processing using the Citadel 2000 Table Top Processor
Tissue is processed overnight.
Note
Ethanols and Xylenes are at room temperature and paraffin is at 58-60°C
Note
Paraffin is Fisherbrand Histoplast PE Cat.#: 22900700
Cassettes are submersed in 50% EtOH at the end of the day.
Processor programmed to start overnight and finish the following morning.
Tissue blocks are transferred to a vacuum paraffin bath.
Tissue blocks are then embedded and placed in 4°C wrapped in foil.