Anesthetize the mouse. The following settings should be used for anesthesia during induction: 3-4% isoflurane, 100% oxygen, 1.5-2 liter per minute (LPM) flow rate. The following settings should be used for maintenance: 2-3.5% isoflurane, 100% oxygen, 1.5-2 LPM. Mouse should be continually monitored for regular breading and adequate anesthetic depth via toe pinch reflex. Anesthesia levels should be adjusted if breathing becomes to fast or slow, or if the mouse is responsive to toe pinch.