Protocol Citation: ccherry 2023. JMN-MSMP Muscle RNA Isolation. protocols.io https://protocols.io/view/jmn-msmp-muscle-rna-isolation-czpkx5kw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 07, 2023
Last Modified: September 07, 2023
Protocol Integer ID: 87500
Abstract
Isolation of RNA from muscle samples
Isolation of RNA
Isolation of RNA
Muscle sections were submerged in Trizol Reagent (Thermo Fisher Scientific), minced, and grinded.
Subsequent RNA isolation was performed using the RNAeasy Mini Kit (Qiagen) following manufacturers protocol.