Aug 30, 2024

Public workspaceJAX-Sen: Collection and shipment of specimen for Visium Spatial Transcriptomics (vST/Visium ST) V.2

DOI
dx.doi.org/10.17504/protocols.io.36wgqn193gk5/v2
  • Laura Robinson1,
  • Susan Sheehan1,
  • Gaven Garland1,
  • Ron Korstanje1
  • 1The Jackson Laboratory, Bar Harbor, ME, USA
  • Cellular Senescence Network (SenNet) Method Development Community
Harshpreet Chandok
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DOI: dx.doi.org/10.17504/protocols.io.36wgqn193gk5/v2
Protocol Citation: Laura Robinson, Susan Sheehan, Gaven Garland, Ron Korstanje 2024. JAX-Sen: Collection and shipment of specimen for Visium Spatial Transcriptomics (vST/Visium ST). protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqn193gk5/v2Version created by Harshpreet Chandok
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 28, 2024
Last Modified: August 30, 2024
Protocol Integer ID: 106764
Keywords: JAX-Sen
Funders Acknowledgement:
National Institute on Aging (NIA) JAX-Sen Senescence Tissue Mapping Center
Grant ID: U54 AG079753
Abstract
These samples are part of the JAX-Sen project in the SenNet Consortium. Here we provide details on specimen collection and shipment procedures for Visium Spatial Transcriptomics (vST/Visium ST).
Reagents and Materials:
Reagents and Materials:
  • 10% NBF fixative
  • Tweezers
  • Appropriate container for fixing
Quality Key Points:
Quality Key Points:
  • The tissue specimen should be always kept at 4 degrees Celsius and RNase-free.
  • It is crucial to not store the tissue specimen at RT to avoid any cell death, and tissue and/or RNA degradation. 
Procedure:
Procedure:
Collection/Harvest:
Animal was euthanized via cervical dislocation. 

Animal was pinned to a necropsy tray which remained on wet ice throughout the harvest. 
Animal was perfused with 20ml cold PBS.
Heart, pancreas, and kidney was collected and the specimen was fixed in 10-20 X volume of 10% NBF fixative for 24-48 hours at room temperature.
Samples were kept at 4 degrees Celsius until submission to histology for processing and embedding.