Dec 18, 2024
JAX-Sen: Chromium GEM-X Single Cell 3' v4 (CG000731 | Rev B)
- Sandra L. Daigle1,
- Elise T. Courtois2,
- William F. Flynn2,
- Marie Jacques Martine Seignon2,
- Greggory Perry1,
- Jessica D. S. Grassmann2
- 1Single Cell Biology Lab, The Jackson Laboratory, USA;
- 2The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Sandra L. Daigle, Elise T. Courtois, William F. Flynn, Marie Jacques Martine Seignon, Greggory Perry, Jessica D. S. Grassmann 2024. JAX-Sen: Chromium GEM-X Single Cell 3' v4 (CG000731 | Rev B). protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4wz28vo5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 17, 2024
Last Modified: December 18, 2024
Protocol Integer ID: 115817
Keywords: JAX-Sen
Funders Acknowledgements:
National Institute on Aging (NIA) JAX-Sen Senescence Tissue Mapping Center Grant
Grant ID: U54 AG079753
Abstract
10x Genomics Chromium Single Cell Expression protocol for library construction.
Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.
Procedure
Procedure
Complete single cell or nuclei isolation and 10x fixation prior to starting this protocol
Library Preparation
Library Preparation
The protocol follwed for the library preparation can be referred to the link: https://cdn.10xgenomics.com/image/upload/v1725314293/support-documents/CG000731_ChromiumGEM-X_SingleCell3v4_UserGuide_RevB.pdf