HAMS/F12 medium (Thermo Fisher, 11765054)
Trypsin (Alfa Aesar, J63993-09)
EDTA (Sigma-Aldrich, E9884-100G)
Collagenase V (Sigma-Aldrich, C9263-100MG)
Heated magnetic stirrer (VWR, 76447-030) (or equivalent)
Narrow orifice 10mL serological pipette (VWR, 89130-898) (or equivalent)
Advanced DMEM/F12 medium (Life Technologies 12634-010)
Matrigel (Corning 356231)
Blunt 200 µl pipette tips (Fisher 02-707-134) (or equivalent)
N2 (Life Technologies, 17502-048)
B27 (Life Technologies, 17504-044)
Primocin (InvivoGen, ant-pm-1)
L-glutamine (Life Technologies, 35050-061)
hR-spondin1 (R & D systems, 4645-RS-100)
hFGF2 (Peprotech, 100-18C)
Nicotinamide (NTM)(Sigma, N0636-100G
Y-27632(Sigma, Y0503-1MG)
PGE2 (R & D systems, 22-961-0)
FBS (heat inactivated) (Cytiva HyClone, SH30070.03)
Advanced DMEM/F12 (Life Technologies, 12634-010)