Stock | Name | Final Conc. | Fold Dilution (x) | Total Vol. (ul) | |
1 | M Sucrose | 0.26 | 3.87 | 51706.50 | |
2 | M KCl | 0.03 | 77.36 | 2585.33 | |
1 | M MgCl2 | 0.01 | 193.40 | 1034.13 | |
0.75 | M Tricine-KOH pH 7.8 | 0.02 | 36.26 | 5515.36 | |
- | Water | - | - | 139158.69 |
Stock | Name | Final Conc. | Fold Dilution (x) | Total Vol. (ul) | |
2 | M KCl | 0.15 | 13.33 | 7500.00 | |
1 | M MgCl2 | 0.03 | 33.33 | 3000.00 | |
0.75 | M Tricine-KOH, pH 7.8 | 0.12 | 6.25 | 16000.00 | |
- | Water | - | - | 73500.00 |
Stock | Name | Final Conc. | Fold Dilution (x) | Total Vol. (ul) | |
- | Diluent Buffer | 1 | - | 8333.33 | |
60 | % Iodixanol | 50 | 1.20 | 41666.67 |
Stock | Name | Final Conc. | Fold Dilution (x) | Total Vol. (ul) | |
1 | M Tris-HCl pH 7.5 | 0.01 | 100.00 | 5000.00 | |
5 | M NaCl | 0.01 | 500.00 | 1000.00 | |
1 | M MgCl2 | 0.003 | 333.33 | 1500.00 | |
- | Water | - | - | 492500.00 |
Stock | Name | Final Conc. | Fold Dilution (x) | Vol per 12 samp. (ul) | |
1.0341 | x HB Stable Solution | 1 | 1.03 | 24175.00 | |
1 | M DTT | 0.001 | 1000.00 | 25.00 | |
500 | mM Spermidine | 0.5 | 1000.00 | 25.00 | |
150 | mM Spermine | 0.15 | 1000.00 | 25.00 | |
10 | % NP40 | 0.3 | 33.33 | 750.00 | |
- | cOmplete Protease Inhibitor | - | - | 0.50 Tablets |
Stock | Name | Final Conc. | Fold Dilution (x) | Vol per sample (ul) | |
- | 1x Homog. Buffer Unstable | - | - | 240.00 | |
50 | % Iodixanol Solution | 30 | 1.67 | 360.00 |
Stock | Name | Final Conc. | Fold Dilution (x) | Vol per sample (ul) | |
- | 1x Homog. Buffer Unstable | - | - | 120.00 | |
50 | % Iodixanol Solution | 40 | 1.25 | 480.00 |
Stock | Name | Final Conc. | Fold Dilution (x) | Vol per sample (ul) | |
- | ATAC-RSB | - | - | 2970.00 | |
10 | % Tween-20 | 0.1 | 100.00 | 30.00 |
Reagent | Vol per sample (ul) | |
H2O | 5 | |
PBS | 16.5 | |
2x TD Buffer | 25 | |
1% Digitonin | 0.5 | |
10% Tween-20 | 0.5 | |
Tn5 Transposase | 2.5 |
Item | Supplier | Cat Number | |
Eppendorf 2 ml Lo-Bind tubes | Sigma | Z666556-250EA | |
Eppendorf 1.5 ml Lo-Bind tubes | Sigma | Z666548-250EA | |
Nunc cryovials | Thermo | 375418PK | |
Iodixanol (comes at 60%) | Sigma | D1556-250ML | |
Sucrose | Sigma | S7903-250G | |
NP40 | Roche (Sigma) | 11332473001 | |
Tricine | Sigma | T0377-25G | |
Potassium Hydroxide (KOH) | Sigma | P5958-250G | |
cOmplete Protease Inhibitors | Roche | 11697498001 | |
MgCl2 | Ambion (Thermo) | AM9530G | |
KCl | Ambion (Thermo) | AM9640G | |
DTT | Thermo | R0861 | |
Spermidine | Sigma | S2501 | |
Spermine | Sigma | S3256-1G | |
70 um Flowmi cell strainers | Fisher | 03-421-228 | |
70 um bucket-style cell strainers | BD Falcon | 352350 | |
Tris-HCl pH 7.5 | Invitrogen | 15567-027 | |
NaCl | Ambion (Thermo) | AM9759 | |
Tween 20 | Roche (Sigma) | 11332465001 | |
H2O | Invitrogen | 10977-015 | |
Dounce Tissue Grinder Set | Sigma | D8938-1SET | |
INCYTO Disposable hemocytometers | Fisher | 22-600-100 | |
BAM Banker | Wako Chemicals | 302-14681 | |
RiboLock | Thermo | EO0384 | |
0.22 um PVDF Filter Units (500 ml) | Millipore | SCGVU05RE | |
0.22 um PVDF Filter Units (50 ml) | Millipore | SE1M179M6 | |
Tn5 Transposase (TDE1) | Illumina | 15027865 | |
2x TD Tagment DNA Buffer | Illumina | 15027866 |