Mar 17, 2025
Version 2
Isolation of Nuclei from Brain Tissue Using Gradient Centrifugation for Myelin Depletion for 10x Genomics Platform V.2
- Allen Institute1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute 2025. Isolation of Nuclei from Brain Tissue Using Gradient Centrifugation for Myelin Depletion for 10x Genomics Platform. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvok1exl4o/v2Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 06, 2024
Last Modified: March 18, 2025
Protocol Integer ID: 124531
Keywords: 10X Genomics, snRNAseq, Multiome
Abstract
Protocol is used for isolation of nuclei from frozen brain tissue or
thawed and microdissected brain tissue sections for RNA-seq analysis.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number
UM1MH130981. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health
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