Many institutes have old fixed slides for microbiological purposes. These slides have valuable material for a particular
pathogen, which can be used for molecular analysis e.g., as reference control during conventional as well as real time PCR along with the gene sequencing. The problem is that user has fixed slides with spots, but they have to develop and standardize the method of isolation leading to wastage of time. Therefore, in this procedure, we are going to show step by step method to isolate DNA with Genekam DNA isolation (mini column) kit from slides. In our laboratory, we have used this method for DNA isolation from microscopic glass slides, which are many years old, very successfully. Sometimes, many laboratories have slides with fixed material for conducting the fluorescent microscopy or slides for conducting student practical, where our protocol can be used. This method is also suitable for histological as well as pathological slides for isolation of tissue DNA for biomarkers analysis. In parasitology, it is common to make the blood smears to examine them under microscope for the presence of parasites in the blood e.g. Plasmodium falciparum, hence such slides can be sent to reference laboratories for molecular analysis, where the reference lab can isolate the DNA with the method in this protocol as this is an innovative idea.