Nov 02, 2023
Isolating Fusarium from plant material
This protocol is a draft, published without a DOI.
- Jenna Moore1
- 1Ohio State University, Department of Plant Pathology
Protocol Citation: Jenna Moore 2023. Isolating Fusarium from plant material. protocols.io https://protocols.io/view/isolating-fusarium-from-plant-material-c4edyta6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working.
Created: November 01, 2023
Last Modified: November 02, 2023
Protocol Integer ID: 90277
Abstract
Protocol for isolating Fusarium (SDS) from soybean plants. A fresh sample is best. Incubating the root sample at 5-10 degrees Celsius encourages sporulation. Samples should be processes no later than 10 days after receiving for best isolation results. Once a pure culture is achieved, find next protocols for single spore culture.
Isolating Fusarium from plant material
Isolating Fusarium from plant material
5d 0h 7m
Wash root system.
Using pruners, remove small sections of the taproot and place into sterile petri dish. If necessary, use a scalpel or blade to cut sections into smaller pieces.
Rinse root sections in 20% bleach for 00:01:00 , followed by sterile water for 00:01:00
2m
Place on sterile paper towels until dried (approximately00:05:00 ).
5m
Plates 4-5 root sections onto APDA (with antibiotics). Incubate at room temperature. Room temperature
Observe growth after 120:00:00 days and hyphal tip transfer colonies onto fresh APDA. Note: F. virguliforme is slow growing.
5d
After initial observations are recorded, use pruners to cut and discard aboveground plant material (keep material from soil-line down).