Aug 09, 2023
Ionpath MIBI (Multiplexed Ion Beam Imaging) Protocols -- University of Minnesota TMCs
Protocol Citation: Mickayla DuFresne-To: Ionpath MIBI (Multiplexed Ion Beam Imaging) Protocols -- University of Minnesota TMCs. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8jyp8g2w/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 03, 2023
Last Modified: August 09, 2023
Protocol Integer ID: 81376
Funders Acknowledgement:
NIH
Grant ID: 1U54AG07041-01
NIH
Grant ID: 1U54AG079754-01
Abstract
Link to information regarding Ionpath MIBI as used by the University of Minnesota
Slides and Reagents from Ionpath for MIBI
Slides (Cat#: 567001): specially formulated, high-purity gold-coated slides
Reagents
MIBI 20X TBS-T (Cat#: 567005)
MIBI 10X PBS (Cat#: 567004)
MIBI 10X Tris pH8.5 (Cat#: 567003)
MIBI diH2O (Cat#: 567002)
Current Ionpath company Conjugated Antibody information for human FFPE tissue and mouse FFPE or frozen tissue: https://www.ionpath.com/conjugated-antibodies/
Ionpath Conjugation kits:
Overview of the MIBI staining protocol with Ionpath reagents and slides
1. Prepare and bake the slides
2. Deparaffinization and Rehydration
3. Heat-induced Antigen retrieval
4. Wash with MIBI 20X TBS-T (Cat#: 567005)
5. Blocking
6. Antibody Staining (Metal-conjugated Abs)
7. Fixation and Dehydration
8. Dry and Store the slides if not processing on scope right away.