Oct 19, 2022
Version 4
Invertebrate bulk sample metabarcoding protocol collection V.4
- 1University of Duisburg-Essen, Aquatic Ecosystem Research
Collection Citation: Dominik Buchner 2022. Invertebrate bulk sample metabarcoding protocol collection . protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlkw4n6l5r/v4Version created by Dominik Buchner
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: October 18, 2022
Last Modified: October 19, 2022
Collection Integer ID: 71476
Keywords: metabarcoding, malaise trap, lysis, DNA extraction, PCR cleanup, PCR normalization
Abstract
This is a collection of protocols currently used in the LeeseLab to perform invertebrate bulk sample metabarcoding. Feel free to contact us if any questions arise.
Files
Protocol
Name
Sample preparation and lysis of homogenized malaise trap samples
Version 1
Dominik BuchnerUniversity of Duisburg-Essen, Aquatic Ecosystem Research
Protocol
Name
Guanidine-based DNA extraction with silica-coated beads or silica spin columns
Version 2
Dominik BuchnerUniversity of Duisburg-Essen, Aquatic Ecosystem Research
Protocol
Name
PCR cleanup and size selection with magnetic beads
Version 2
Dominik BuchnerUniversity of Duisburg-Essen, Aquatic Ecosystem Research
Protocol
Name
PCR normalization and size selection with magnetic beads
Version 2
Dominik BuchnerUniversity of Duisburg-Essen, Aquatic Ecosystem Research