Nov 02, 2023
Intrinsic water use efficiency estimate: an isotopic method
- Flavie Gerle1,
- Pauline Malherbe2,
- Christelle Boisselet1,
- David Lafleuriel1,
- Julien Godfroy2,3,
- Pierre Lochin2,
- Baptiste Marteau2,
- Hervé Piegay2,
- Sara Puijalon1,
- Antoine Vernay1
- 1Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR 5023 LEHNA, F-69622, Villeurbanne, France;
- 2UMR 5600 EVS, ENS de Lyon, F-69342 Lyon, France;
- 3National Research Institute of Science and Technology for Environment and Agriculture, Research Unit Mountain Ecosystems, University Grenoble Alpes, 2 rue de la Papeterie, F-38402 St-Martin-d’Hères
Protocol Citation: Flavie Gerle, Pauline Malherbe, Christelle Boisselet, David Lafleuriel, Julien Godfroy, Pierre Lochin, Baptiste Marteau, Hervé Piegay, Sara Puijalon, Antoine Vernay 2023. Intrinsic water use efficiency estimate: an isotopic method . protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp3xk8vzp/v1
Manuscript citation:
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 02, 2023
Last Modified: November 02, 2023
Protocol Integer ID: 90313
Keywords: phloem, d13C, intrinsic water use efficiency, tree
Funders Acknowledgement:
OHM Vallée du Rhone
Grant ID: ANR-11-LABX-0010
FR BioEEnViS
Abstract
Protocol summary and key steps
This protocol describes in detail how to calculate the intrinsic water use efficiency (WUEi) from 13C measurements in phloem samples collected at breast height in trees. Basically, the isotopic signature of photosynthetized carbohydrates (13C/12C) in the phloem sap inform on the assimilation condition: a higher (13C/12C) is associated with a lower transpiration rate, increasing heavy isotope (13C) use by the plant (Dawson et al. 2002). A higher 13C/12C in the phloem content reflect a higher proportion of assimilated C compared to transpiration rate, in other words, a higher WUEi. The method described here is based on the isotopic discrimination of δ13C in trees and considers ecophysiological processes of stomatal and mesophyll conductance (gs and gm, respectively). The protocol aims to provide the operator with a step-by-step method from the collection of phloem samples in the field to the
final calculation. The protocol is already described in the scientific literature (Klein et al. 2016; Seibt et al. 2008; Vernay et al. 2020) but this document addresses more practical and technical details that a practitioner might expect. WUE (water use efficiency) corresponds to plant productivity or the productivity of an organ of the plant regarding the amount of water used to produce this fresh matter (Théroux Rancourt 2014). At an individual scale, WUE considers a ratio of the biomass produced compared to the amount of water used to produce this biomass. At the leaf level, WUEi (intrinsic WUE) determines the ratio of CO2 assimilation per amount of water transpired and can take into account the micro-environment of the leaf, by considering gs/gm to be more precise (Vialet-Chabrand 2013).
Attachments
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Antoine VERNAY
Protocol references
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