Jun 16, 2023

Public workspaceIntraperitoneal L-dopa injection in mice

DOI
dx.doi.org/10.17504/protocols.io.81wgbxd51lpk/v1
  • 1Northwestern University, Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 20815
natalia.lopezgonzalezdelrey
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DOI: dx.doi.org/10.17504/protocols.io.81wgbxd51lpk/v1
Protocol Citation: natalia.lopezgonzalezdelrey, Zachary Gaertner 2023. Intraperitoneal L-dopa injection in mice. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbxd51lpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 12, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 83209
Keywords: ASAPCRN, L-dopa, Parkinson's disease, benserazide
Funders Acknowledgement:
Aligning Science Across Parkinson's [ASAP-020600] through the Michael J. Fox Foundation for Parkinson's Research (MJFF)
Grant ID: ASAP-020600
Abstract
Intraperitoneal L-dopa injection in mice
Preparation
Preparation
1h
1h
Mice were acclimated to the room for Duration01:00:00

1h
L-dopa preparation
L-dopa preparation
1h
1h
Prepare the L-dopa mix fresh every day.
Prepare a master mix of: 12.5mg/kg L-dopa and 12.5mg/kg benserazide dissolved in sterile saline with 0.25% citrate (wt/vol). Calculate grams depending on number of mice to be injected. Using vortex will dissolve the solution easier.
L-dopa injection
L-dopa injection
5m
5m
Mice are injected intraperitoneally with the 12.5mg/kg L-dopa and 12.5mg/kg benserazide solution.
Behaviour
Behaviour
1d
1d
One hour post injection, mice were tested using the appropiate behavior protocol.