Dec 14, 2023

Public workspaceINTRAMUSCULAR PFF INJECTION PROTOCOL

DOI
dx.doi.org/10.17504/protocols.io.n2bvj6j75lk5/v1
  • 1University of Minnesota
Jane Balster
Open access
DOI: dx.doi.org/10.17504/protocols.io.n2bvj6j75lk5/v1
Protocol Citation: LEE LAB RESEARCH LABORATORY, Michael Lee 2023. INTRAMUSCULAR PFF INJECTION PROTOCOL. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj6j75lk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 04, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 63887
Keywords: intramuscular, PFF injection, ASAPCRN
Funders Acknowledgement:
ASAP
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Abstract
This protocol details the intramuscular PFF injection procedure.
Attachments
452-956.docx
452-956.docx
17KB
Materials
Materials:
  • PFF: 1 mg/mL
  • Sonication: 1 Second (on/off); 120 Seconds, Amplitude – 20%
  • Dose: 5 mL/side
  • Dental Needle (27G)
  • Hamilton Syringe (x2)
  • Sterile Saline
  • Alcohol wipes
  • Procedure record forms
  • Tubing
  • Heating pads (x2)
  • Clean cages
  • Isoflurane and desiccator
Procedure
Procedure
Clean the procedure hood.
Turn on the heating pad and cover it with a blue procedure pad.
Connect dental needle with tubing.
Fill up PBS into the tubing using a small syringe.
Insert the Hamilton Syringe into the tube for 2-3 mm.
Create air gap between saline solution and PFF solution.
Keep mouse inside desiccator w/ isoflurane until breath becomes long and slow.
Two separate people should inject each respective Bicep femoralis of the mouse simultaneously.
Perform injection with animal on warm pad and keep clean recovery cage on separate heating pad until animal recovers.
Wipe needle with alcohol wipes between sample draw/mouse injection.