Oct 20, 2023
Intestinal transit time
- 1Vall d'Hebron Research Institute
Protocol Citation: Marina Lorente Picón, Ariadna Laguna, Miquel Vila 2023. Intestinal transit time. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyje1plx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 19, 2023
Last Modified: November 20, 2023
Protocol Integer ID: 89595
Abstract
Intestinal transit time measurement in mice
Prepare red dye (Carminered dye, Sigma #C1022) in 0,5% methylcellulose (Sigma #M7027).
Take the animal and administer the red dye by oral gavage.
After oral gavage, individualize the animals in separate boxes without bedding.
Monitor the animal boxes for a total time of 4h.
Register the time when the animals defecate the first dyed fece (stained red).
Represent the time until first dyed fece as the transit time of the animals.