Jun 21, 2022
Inserting RFID into rats
- a.beeson1,
- Leah Solsberg-Woods2,
- Gabriel J Barrero1,
- Oksana Polesskaya1,
- Abraham Palmer1
- 1University of California, San Diego;
- 2Wake Forest University
Protocol Citation: a.beeson, Leah Solsberg-Woods, Gabriel J Barrero, Oksana Polesskaya, Abraham Palmer 2022. Inserting RFID into rats. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzyxzrlx1/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 09, 2022
Last Modified: June 21, 2022
Protocol Integer ID: 59274
Abstract
Insert RFID chips into rats
Guidelines
Save all old cage cards for verification of DOB. Weaned Pups will be housed in Coleman B113 for at least 2 days before transported to TSRB 155.
Materials
. Syringe with needle and non-sterile RFIDs (soaked in 75 % volume EtOH Overnight or longer, then air dry before use)
. Funnel bag
Disinfect needle with 70% EtOH and air dry
Carefully install one RFID into the clean needle
scruff rat skin on the back of neck with fingers
Inset the sharp tips of the needle under the skin, target the back of the neck, close to the head.
Release the RFID by pushing the plunger of the syringe
Scan and make sure the RFID is under the skin
upload the scanned RFID into a spreadsheet