Jun 22, 2023

Public workspaceInduced Pluripotent Stem Cell Reagents

DOI
dx.doi.org/10.17504/protocols.io.rm7vzxnorgx1/v1
  • 1Jason Waligorski, Collin Kremitzski, Purva Patel
Mallory Wright
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DOI: dx.doi.org/10.17504/protocols.io.rm7vzxnorgx1/v1
Document Citation: Mallory Wright 2023. Induced Pluripotent Stem Cell Reagents. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzxnorgx1/v1
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: June 21, 2023
Last Modified: June 22, 2023
Document Integer ID: 83826
Abstract
reagents used to maintain our IPSC


ReagentRockiSTEMCELL Technologies Inc.Catalog #72304

Note
iPSCs like being in clusters. Single cell iPSCs tend to die. ROCKi prevents that from happening

Need a 10mM stock: dilute 5mg of the powder in 1.56 ml nuclease free water. Aliquot into smaller quantities. Store in -20C.

RevitaCell is a good alternative, either can be used


ReagentmTeSR plus media kitSTEMCELL Technologies Inc.Catalog #100-0276 Temperature4 °C
ReagentAntibiotic/Antimycotic (100X)Thermo Fisher ScientificCatalog #15240062 Temperature-20 °C
ReagentReLeSRSTEMCELL Technologies Inc.Catalog #05872 TemperatureRoom temperature
ReagentTrypLE Select (1X) no phenyl redThermo Fisher ScientificCatalog # 12563-011 TemperatureRoom temperature
ReagentMatrigel Matrix hESC qualifiedCorningCatalog #354277 Temperature-20 °C
ReagentDMEM/F12 (1:1)ThermofisherCatalog #11320033 Temperature4 °C
ReagentCryoStor CS10 -100mlSTEMCELL Technologies Inc.Catalog #07930 Temperature4 °C
ReagentDBPS -/- (without calcium / magnesium)Thermo Fisher ScientificCatalog #14190144 TemperatureRoom temperature