Induced pluripotent stem cell (iPSC)-derived neurons are an important tool for studying diverse types of neurodegenerative disorders, including Alzheimer’s Disease, Parkinson’s disease, and related dementias. Understanding the molecular and cellular mechanisms associated with these diseases is an important step in developing new therapeutic targets. Here we describe a robust differentiation protocol in which we expressed the human neurogenin 2 (NGN2) transcription factor under a tetracycline-inducible promoter as previously described (Fernandopulle et al. 2018), with several modifications and using a PiggyBac system for delivery. This differentiation protocol yields high percentages of cortical neuron markers.