Add/Remove scanning channels by clicking the green + or red X to the right of the Name, Plate/Slide/Objective fields. Rename each channel (originally labeled with color(blue, green, red, far red, orange) to the corresponding stain (Hoechst, Mitotracker Deep Red or Green, Tubulin Tracker Deep Red, Cell mask orange/green, etc). Make sure channels correspond to the correct laser. Examples include: Hoechst=405, Cell Mask Orange=561, Mitotracker Green=488, Tubulin Tracker Deep Red=642. Edit each scanning channel (right click, select edit) to reflect the correct target type. For example, the blue channel name will be changed to hoechst. Then edit the channel to adjust the target type to Nuclei. Target type options: Nuclei, Cells, Organelles, Other.