Samples are resuspended in water or 1% (v/v) TX-100 in PBS. For TX-100 lysis, cells incubated on ice for 15 mins and debris/nuclei removed by centrifugation at 17, 000 x g, 10 min, 4 °C. Supernatant containing GBA enzyme placed in fresh tube. All samples are sonicated in water bath for 1 minute. Protein concentration measured with BCA protein assay.