Here are a few controls you can use to ensure assay is working correctly:
o Negative control (primary antibody only)
§ This slide receives 0.05% PBS-T in place of secondary antibody
o Negative control (secondary antibody only)
§ This slide receives 1% BSA in PBS in place of diluted primary antibody
§ If performing staining across multiple batches, include serial sections of one tissue in each batch that has positive staining for Salmonella
Parameters for some steps (e.g. antibody incubations, antigen retrieval, chromogen incubations, counterstaining) may need to be further optimized for different tissues or targets.