Pipettes/pipette tips – volumes ranging between 2-1000 uL
Drying oven (able to reach & hold 60℃)
Fume hood
Slide staining tray (e.g. Simport M920-2)
Tissue-Tek Vertical 24 slide rack (American Master Tech Scientific LWS2124)
Tissue-Tek Staining Dishes (American Master Tech Scientific LWS20WH)
Tissue-Tek Clearing Agent Dishes, xylene resistant (American Master Tech Scientific LWS20GR)
Decloaking Chamber NxGen (Biocare Medical DC2012/DC2012-220V)
Bright field microscope
***For all reagents, refer to MSDS to determine appropriate precautions, personal protective equipment (PPE),
and disposal methods before use***
Distilled water (obtained in-house)
0.05% PBS-Tween (PBS-T), pH 7.35 (made in-house)
Xylenes (Macron Fine Chemicals 8668-16)
100% ethanol (Pharmco 111000200)
------------o Dilute with distilled water to make 95%, 85%, and 70% concentrations
------------o 10% NBF (Cancer Diagnostics, Inc. 111) or 4% PFA (Electron Microscopy Sciences 15713)
------------o Co-Detection Target Retrieval Reagents (ACD 323165/323166)
------------o Co-Detection Antibody Diluent (ACD 33160)
Dual Endogenous Enzyme Block (Dako S2003)
Protein Block (Dako X0909)
Anti-Bcl6 antibody; mouse IgG1 clone K112-91; stock concentration 500 ug/mL (BD 561520)
EnVision+ System HRP Labelled Polymer Anti-Mouse (Dako K400111-2)
Liquid DAB+ (Dako K346811-2)
------------o DAB+ Substrate Buffer
------------o DAB+ Chromogen
Gill’s Hematoxylin I (American Master Tech Scientific HXGHE1LT)
Refrax Mounting Medium (Anatech 711)
#1 thickness cover glass (Fisherbrand 12-545-F)