Apply primary antibodies to TH (Abcam Cat# ab76442, RRID:AB_1524535, 1:500), GFP (Thermo Fisher Scientific Cat# A-11122, RRID:AB_221569, Invitrogen, 1:1000), CASR (Abcam Cat# ab79038, RRID:AB_2071489, 1:100) or TYROBP/DAP12 (Abcam Cat# ab283679, 1:100), prepared in 1 % donkey serum in TBS and incubate overnight at 4 °C