License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 01, 2022
Last Modified: March 01, 2022
Protocol Integer ID: 58941
Abstract
This protocol is used to stain cryosectioned mouse brain tissue.
To cryo-sectioned brain tissue, wash with 1X phosphate buffered saline (PBS) for 3x 5-minute washes.
15m
Incubate in blocking buffer for 1 hour at room temperature.