10% Buffered Formalin (Fisher Scientific, SF100-4)
10X Phosphate Buffered Saline (Thermo Fisher Scientific, #28374)
Hydrophobic Barrier Pen (Vector Labs, H-4000)
Avidin/Biotin Blocking Kit (Vector Labs, SP-2001)
10X Power Block Universal Blocking Solution (BioGenex, HK085-5K)
Antibody Diluent Reagent Solution (Thermo Fisher Scientific, 003218)
Double Distilled Water to dilute PBS to 1X
Hoechst 33342 (Thermo Fisher Scientific, 62249)
100% Glycerol (Fisher Scientific, G33)
Serum for Additional Block (Example: Normal Rabbit Serum, Sigma-Aldrich, NS01L-1ML)
Secondary Antibody with Minimal Cross Reactivity
- 100-Slide Storage Box (Fisher Scientific, 03-448-1)
- Kimwipes, 8.4 in x 4.4 in (Fisher Scientific, 06-666)