Apr 18, 2022
Immunofluorescence
- William Hancock-Cerutti1,2,3,4,5,
- Pietro De Camilli1,2,3,5
- 1Departments of Neuroscience and of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA;
- 2Howard Hughes Medical Institute;
- 3Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, Connecticut 06510, USA;
- 4Interdisciplinary Neuroscience Program and MD-PhD Program, Yale University School of Medicine, New Haven, Connecticut 06510, USA;
- 5Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815
Protocol Citation: William Hancock-Cerutti, Pietro De Camilli 2022. Immunofluorescence. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn741mv5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 18, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 60899
Keywords: ASAPCRN
Abstract
This protocol describes the fixation of cultured cells in paraformaldehyde solution and immunofluorescent labeling of mitochondria and DNA.
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