Day 3-4: Fix and label cells
Tips and Troubleshooting:
We recommend wiping down all pipettes and equipment with 10% bleach prior to use.
The optimal fixation method will vary depending on the sample type and the protein of interest. You may need to try a variety of fixation methods to find the best conditions for your target.
The optimal antibody concentration will vary between antibodies. Review the manufacturer's instructions before starting your experiment and consider titrating your antibody to determine the optimal dose.
To ensure that your antibody is both functioning as expected and specific, include a positive control sample that you know expresses the protein, such as cells transfected with a plasmid to express the protein of interest, and a negative control sample such as cells that do not express the protein of interest.