The protocol was published on behalf of the investigators by the SPARC project team.
The University of Toledo Health Science Campus animal care and use committee approved animal care, breeding procedures, and experimental protocols. Animals were housed in an Association of Laboratory animal Care-approved facility with a 12-hour light cycle with food (standard chow) and water ad libitum.
One male VIP-GCAmP reporter mouse, age 4 months, was used in this study. The mouse was purchased from the Jackson Laboratories, and all genotyping was performed using primers recommended by the Jackson Laboratories according to their protocols.