Mar 08, 2024
Illustrated Protocol for Processing Radiometric Videos Using the Software FLIR Tools : An Approach Applied to a Novel Methodology for Lizard Thermal Exchange Rates Studies
- Frederico M Barroso1,2,3
- 1CIBIO, Research Centre in Biodiversity and Genetic Resources, InBIO, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, Vairão 4485-661, Vila do Conde, Portugal;
- 2Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, R. Campo Alegre, s/n, 4169 - 007, Porto, Portugal;
- 3BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, 4485-661 Vairão, Portugal
Protocol Citation: Frederico M Barroso 2024. Illustrated Protocol for Processing Radiometric Videos Using the Software FLIR Tools : An Approach Applied to a Novel Methodology for Lizard Thermal Exchange Rates Studies. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj358xlk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 07, 2024
Last Modified: March 08, 2024
Protocol Integer ID: 96315
Keywords: thermal imaging, radiometric videos, FLIR, thermal video processing, thermal physiology
Funders Acknowledgement:
Fundação Para a Ciência e a Tecnologia
Grant ID: SFRH/BD/147535/2019
Fundação Para a Ciência e a Tecnologia
Grant ID: COVID/BD/153468/2023
Abstract
The novel methodology developed by Mochales-Riaño & Barroso et al. (in press) for experimentally obtaining thermal exchange rates of small lizards through the use of radiometric videos, underpinned the need to devise a thermal video processing protocol to standardize this data collection. This protocol addresses this need as it represents a subsequently optimized version of the processing pipeline used for the aforementioned proof-of-concept study.
This protocol was used to successfully obtain temperature data from several body parts from thermoregulating lizards, enabling internal body temperature to be inferred (as per Barroso et al., 2016 and 2020), as well as providing insights into thermal exchange rates and the patterns of regional heterothermy and temperature redistribution in these animals.
So far, variants of this video analysis protocol have been successfully deployed in several ongoing research projects, on several species of lizards.
Attachments
Image Attribution
All images by the Author, Frederico M Barroso, except the diagram of the lizards head kindly donated by Giulia Simbula.
Guidelines
When processing multiple videos for posterior comparison of data, it is recommended that said processing is done by the same person in order to avoid any operator bias.
Protocol references
Barroso, F. M., Carretero, M. A., Silva, F., & Sannolo, M. (2016). Assessing the reliability of thermography to infer internal body temperatures of lizards. Journal of Thermal Biology, 62, 90–96. https://doi.org/10.1016/j.jtherbio.2016.10.004
Barroso, F. M., Riaño, G., Sannolo, M., Carretero, M. A., & Rato, C. (2020). Evidence from Tarentola mauritanica (Gekkota: Phyllodactylidae) helps validate thermography as a tool to infer internal body temperatures of lizards. Journal of Thermal Biology, 93, 102700. https://doi.org/10.1016/j.jtherbio.2020.102700
Faye, E., Dangles, O., Pincebourde, S. (2016). Distance makes the difference in thermography for ecological studies. Journal of Thermal Biology, 56, 1–9.