Oct 20, 2021

Public workspace(ILLUMINA MENU) Protocols for SARS-CoV-2 Library Prep with ARTIC Primers, Bioinformatic Analysis, and Database Submission V.2

This collection is a draft, published without a DOI.
(ILLUMINA MENU) Protocols for SARS-CoV-2 Library Prep with ARTIC Primers, Bioinformatic Analysis, and Database Submission
  • TOAST CDC, OAMD CDC1
  • 1Centers for Disease Control and Prevention
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Collection CitationTOAST CDC, OAMD CDC 2021. (ILLUMINA MENU) Protocols for SARS-CoV-2 Library Prep with ARTIC Primers, Bioinformatic Analysis, and Database Submission . protocols.io https://protocols.io/view/illumina-menu-protocols-for-sars-cov-2-library-pr-by8rpzv6Version created by Technical Outreach and Assistance for States Team
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this collection
Created: October 20, 2021
Last Modified: October 20, 2021
Collection Integer ID: 54257
Keywords: SARS-CoV-2, COVID-19, sequencing, bioinformatics, Illumina, protocols for sar, office of advanced molecular detection, submission of sar, advanced molecular detection, used protocol, sar, territorial lab, bioinformatic analysis, centers for disease control, laboratory
Disclaimer
The opinions expressed here do not necessarily reflect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affiliated. The protocol content here is under development and is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Abstract
This collection of protocols covers library preparation and sequencing on Illumina platforms as well as bioinformatic analysis and submission of SARS-CoV-2 data. These frequently used protocols have been assembled by the Technical Outreach and Assistance for States Team (TOAST) and the Office of Advanced Molecular Detection (OAMD) at the Centers for Disease Control and Prevention for use by participating State, Tribal, Local, and Territorial labs for sequencing of SARS-CoV-2. Individual protocols were developed by the protocol authors, and individual protocols should be credited accordingly.

These documents are intended for laboratories designated by TOAST and should not be widely or openly disseminated.

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The opinions expressed here do not necessarily reflect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affiliated. The protocol content here is under development and is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Files
Protocol
Icon representing the file SARS-CoV-2 Sequencing on Illumina MiSeq Using ARTIC Protocol: Part 1 - Tiling PCR
Name
SARS-CoV-2 Sequencing on Illumina MiSeq Using ARTIC Protocol: Part 1 - Tiling PCR
Version 1
, Theiagen Genomics
Joel SevinskyTheiagen Genomics
Protocol
Icon representing the file SARS-CoV-2 Sequencing on Illumina MiSeq Using ARTIC Protocol: Part 2 - Illumina DNA Prep Protocol
Name
ForkSARS-CoV-2 Sequencing on Illumina MiSeq Using ARTIC Protocol: Part 2 - Illumina DNA Prep Protocol
Version 1
,
Joseph C Madden
Protocol
Icon representing the file SARS-CoV-2 Sequencing on Illumina MiSeq Using ARTIC Protocol: Part 2 - Illumina DNA Flex Protocol
Name
SARS-CoV-2 Sequencing on Illumina MiSeq Using ARTIC Protocol: Part 2 - Illumina DNA Flex Protocol
Version 1
, Theiagen Genomics
Joel SevinskyTheiagen Genomics
Protocol
Icon representing the file Titan Illumina PE SARS-CoV-2 Strain Characterization Workflow for the Terra Platform
Name
Titan Illumina PE SARS-CoV-2 Strain Characterization Workflow for the Terra Platform
Version 2
, Centers for Disease Control and Prevention
Technical Outreach and Assistance for States TeamCenters for Disease Control and Prevention
Protocol
Icon representing the file DRAGEN COVID Lineage App SARS-CoV-2 Strain Characterization on the Illumina BaseSpace Platform
Name
DRAGEN COVID Lineage App SARS-CoV-2 Strain Characterization on the Illumina BaseSpace Platform
Version 2
,
ycm6
Protocol
Icon representing the file Cecret Workflow for SARS-CoV-2 Assembly and Lineage Classification
Name
Cecret Workflow for SARS-CoV-2 Assembly and Lineage Classification
Version 3
,
ycm6
Protocol
Icon representing the file Titan-gc SARS-CoV-2 Strain Characterization Workflow for Bioconda
Name
ForkTitan-gc SARS-CoV-2 Strain Characterization Workflow for Bioconda
Version 3
, Centers for Disease Control and Prevention
Michael WeigandCenters for Disease Control and Prevention
Protocol
Icon representing the file Mercury Sequence and Sample Metadata Prep for Submission Workflow on the Terra Platform
Name
Mercury Sequence and Sample Metadata Prep for Submission Workflow on the Terra Platform
Version 3
, Centers for Disease Control and Prevention
Technical Outreach and Assistance for States TeamCenters for Disease Control and Prevention
Protocol
Name
SARS-CoV2 GISAID submission protocol
Version 3
, University of Oxford
Nabil-Fareed AlikhanUniversity of Oxford
Protocol
Icon representing the file SARS-CoV-2 NCBI submission protocol: SRA, BioSample, and BioProject
Name
ForkSARS-CoV-2 NCBI submission protocol: SRA, BioSample, and BioProject
Version 3
, US Food and Drug Administration
Ruth TimmeUS Food and Drug Administration
Protocol
Icon representing the file SARS-CoV-2 NCBI consensus submission protocol: GenBank
Name
SARS-CoV-2 NCBI consensus submission protocol: GenBank
Version 2
, US Food and Drug Administration
Ruth TimmeUS Food and Drug Administration
Protocol
Icon representing the file Augur/Auspice/UShER SARS-CoV-2  Cluster Detection Workflow for the Terra Platform
Name
ForkAugur/Auspice/UShER SARS-CoV-2 Cluster Detection Workflow for the Terra Platform
Version 4
,
Anusha Ginni