Fluorescence intensity (FI) assessments and colocalizations were carried out using a confocal laser scanning microscope LEICA TCS-NT (Version 2.5, Build 1227, Leica Microsystems GmBH, Heidelberg, Germany, equiped with image analysis software), with an argon/krypton laser using 10 X, 20 X, and 40 X and 100 X (oil) immersion objectives. Immunofluorescent images were acquired by sequential scanning of 12–16 serial optical sections74. Three‐dimensional reconstructions from z‐series were used to verify colocalization in the x–y, y–z, and x–z planes. Serial fluorescent images were captured in randomly selected areas and the number of labeled cells per field was manually counted, and cell counts obtained averaged (mean ± SEM).