Aug 09, 2022
Version 2
IB BIO SL IA M23 - A study on the conditions that affect yeast fermentation V.2
- 1Universitat de Girona
Protocol Citation: Mar Roca Cugat 2022. IB BIO SL IA M23 - A study on the conditions that affect yeast fermentation . protocols.io https://dx.doi.org/10.17504/protocols.io.261geny6jg47/v2Version created by Mar Roca Cugat
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 09, 2022
Last Modified: August 09, 2022
Protocol Integer ID: 68407
Keywords: biology, fermentation, yeast, ib, ia
Abstract
This protocol is designed to aid me in the research for my International Baccalaureate Biology Internal Assignment
Guidelines
None
Materials
- Flask (3 mouths)
- Thermometer
- Holed plugs x2
- Glass tube
- Rubber tube
- Gas recovery flask
- Basin
- Water
- Sugar
- Yeast
Safety warnings
None
Preparation
Preparation
5m 30s
5m 30s
Check that all reagents and materials are ready.
30s
Assemble the instrumentation
5m
One end of the flask must be plugged with a holed stopper and a thermometer.
The other end should be plugged with a holed stopper and a glass tube.
The middle opening should be covered with plastic and secured with a rubber band.
A long and shallow recipient must be filled with water as much as possible. The gas recovery flask should also be filled with water as much as possible. Then, the latter should be inverted and suspended inside the former.
The tube should be connected from the glass tube to the gas recovery flask.
The block of compressed yeast must be cut in 4 parts
Processing
Processing
30m
30m
Several factors must be checked and accounted for: pressure, temperature and initial weight of the full gas recovery flask (GRF). To check the ambiental conditions, we will use a thermometer and the Google Science Notebook (discontinued) phone application, which uses the phone's integrated barometer to tell the pressure. To measure the GRF weight we will use a scale.
One quarter of the yeast block must be dissolved in sugary water (concentration undetermined, volume undetermined, temperature undetermined). This should then be placed in the flask.
Wait for fermentation: 00:30:00
30m
Weigh the GRF. The difference from the first measurement is the gas generated. Convert it to moles of CO2+H2O for a universal measurement that is constant through different temperatures and pressures.
go to step #4 Repeat as many times as needed